期刊
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
卷 58, 期 10, 页码 3713-3722出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TIM.2009.2019317
关键词
Biomedical signal processing; feature extraction; Listeria; pattern classification; Raman spectroscopy; surface-enhanced Raman scattering (SERS); wavelet transforms
资金
- Canadian Food Inspection Agency
- Natural Sciences and Engineering Research Council of Canada
- Canada Foundation for Innovation
- Ontario Innovation Trust
We investigated the ability to distinguish between six species within the Listeria genus (including the human pathogen Listeria monocytogenes) based on a bacteria sample's surface-enhanced Raman scattering (SERS) spectrum. Our measurement system consists of a portable low-cost Raman spectral acquisition unit and associated signal processing and classification modules. First, Listeria was cultured and then adsorbed onto silver colloidal nanoparticles for SERS measurements. A total of 483 SERS spectra were collected and preprocessed (using a stationary wavelet transform decomposition) to remove noise and baseline artifact. Distinguishing features were extracted by retaining detail wavelet coefficients of significant value across multiple scales. Using a linear classifier in association with leave one out cross-validation, the system achieved maximum classification accuracies of 96.1% (six-category) and 97.9% (two-category, L. monocytogenes versus all others). Dimensionality reduction was used to decrease the number of features from 74 to 5 while maintaining similar classification accuracy. In the future, it is envisioned that a measurement system such as this, which is a combination of low-cost hardware with sophisticated signal processing, could play a complementary role with existing methods in realizing a rapid inexpensive means of identifying food-borne bacterial pathogens.
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