期刊
IEEE TRANSACTIONS ON IMAGE PROCESSING
卷 21, 期 8, 页码 3518-3530出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TIP.2012.2197007
关键词
3D+t mathematical morphology; in-vivo light microscopy; spatio-temporal structuring elements; zebrafish embryogenesis
资金
- European Regional Development Funds
- FP6 NEST Program, AMIT-CDTI [TEC2010-21619-C04-03]
- PRECISION (Spanish Ministry of Science)
- French National Research Agency
- PICATA of the Moncloa Campus of International Excellence, Spain
We propose to directly process 3D + t image sequences with mathematical morphology operators, using a new classification of the 3D + t structuring elements. Several methods (filtering, tracking, segmentation) dedicated to the analysis of 3D + t datasets of zebrafish embryogenesis are introduced and validated through a synthetic dataset. Then, we illustrate the application of these methods to the analysis of datasets of zebrafish early development acquired with various microscopy techniques. This processing paradigm produces spatio-temporal coherent results as it benefits from the intrinsic redundancy of the temporal dimension, and minimizes the needs for human intervention in semi-automatic algorithms.
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