期刊
MARINE ENVIRONMENTAL RESEARCH
卷 110, 期 -, 页码 25-32出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.marenvres.2015.07.019
关键词
Crassostrea gigas; Pacific oyster; Aquaculture; Gulf of California; hsp70; Gene expression
资金
- CONACyT [337347]
The HSP70 proteins are an important element of the response against thermal stress and infectious diseases, and they are highly conserved and ubiquitous. In some species, variations on the hsp70 encoding sequence resulted in intraspecific differential expression, which leads to variations on thermotolerance among individuals. This phenomenon has not been described in the Pacific oyster Crassostrea gigas, which is cultivated in Mexico under temperature conditions highly above the optimal for this species. The present study was aimed to identify associations between hsp70 genotypes and their expression levels in C. gigas. By analyzing a 603 bp fragment from the 3' end of the hsp70 gene, 21 different genotypes with 60 nucleotide polymorphic sites were detected, of which 34 sites were found in heterozygous condition. Although no correlation was found between genotype-expression-season, a minimum expression threshold that should be taken into account as an important feature for a future breeding program is proposed. (C) 2015 Elsevier Ltd. All rights reserved.
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