期刊
MARINE ENVIRONMENTAL RESEARCH
卷 112, 期 -, 页码 61-67出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.marenvres.2015.09.007
关键词
Crustaceans; Glucose transporter; GLUT; Hypoxia; Shrimp; Xenopus oocytes
资金
- Consejo Nacional de Ciencia y Tecnologia, Mexico (CONACYT) [221240]
- Grants-in-Aid for Scientific Research [26850216] Funding Source: KAKEN
In hypoxia conditions, the white shrimp Litopenaeus vannamei shifts its energetic metabolism from aerobic to anaerobic, requiring more glucose uptake into the cells by GLUT proteins. We here report a novel glucose transporter in shrimp. The Lvglut2 cDNA is 2473 bp-long containing an ORF of 1458 bp encoding 486 amino acid residues. The deduced protein has the features of a facilitative sugar transporter. The Lvglut2 gene product tagged with GFP was expressed in the cell membrane of Xenopus oocytes. In the same expression system, untagged LvGLUT2 resulted to be a bidirectional glucose transporter that functions moving glucose down its concentration gradient in and out of the cell. Lvglut2 mRNA is expressed in hepatopancreas while in muscle and gills it was not detected. Hypoxia up-regulates the expression of Lvglut2 transcripts in hepatopancreas. These results provide a better understanding of facilitative glucose transporters and gene regulation during hypoxia in crustaceans. (C) 2015 Elsevier Ltd. All rights reserved.
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