Model Evaluation of Changes in Electrorotation Spectra of Biological Cells After nsPEF Electroporation

Cellular electroporation involves electrically driven dielectric membrane breakdown, and has several bio-medical applications. Electrorotation and changes in its characteristic spectra could be a useful method for monitoring electroporation. Towards this goal, a simulation analyses is presented to probe electrorotation features arising from poration. Breakdown of both outer plasma membrane and inner organelle membranes are included based on a double-shell dielectric model. Our results demonstrate the feasibility of such monitoring, and show that anti-field rotation would be a good indicator of outer plasma membrane rupture, and the degree of poration.