期刊
MARINE DRUGS
卷 13, 期 3, 页码 1255-1266出版社
MDPI AG
DOI: 10.3390/md13031255
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资金
- RFBR [14-04-00885]
- RAS Program MCB
- RSF [14-24-00118]
- Russian Federation [SSch 148.2014.4]
- Russian Science Foundation [14-24-00118] Funding Source: Russian Science Foundation
6-Bromohypaphorine (6-BHP) has been isolated from the marine sponges Pachymatisma johnstoni, Aplysina sp., and the tunicate Aplidium conicum, but data on its biological activity were not available. For the nudibranch mollusk Hermissenda crassicornis no endogenous compounds were known, and here we describe the isolation of 6-BHP from this mollusk and its effects on different nicotinic acetylcholine receptors (nAChR). Two-electrode voltage-clamp experiments on the chimeric alpha 7 nAChR (built of chicken alpha 7 ligand-binding and glycine receptor transmembrane domains) or on rat alpha 4 beta 2 nAChR expressed in Xenopus oocytes revealed no action of 6-BHP. However, in radioligand analysis, 6-BHP competed with radioiodinated alpha-bungarotoxin for binding to human alpha 7 nAChR expressed in GH(4)C(1) cells (IC50 23 +/- 1 mu M), but showed no competition on muscle-type nAChR from Torpedo californica. In Ca2+-imaging experiments on the human alpha 7 nAChR expressed in the Neuro2a cells, 6-BHP in the presence of PNU120596 behaved as an agonist (EC50 ~80 mu M). To the best of our knowledge, 6-BHP is the first low-molecular weight compound from marine source which is an agonist of the nAChR subtype. This may have physiological importance because H. crassicornis, with its simple and tractable nervous system, is a convenient model system for studying the learning and memory processes.
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