期刊
MARINE DRUGS
卷 13, 期 10, 页码 6407-6424出版社
MDPI AG
DOI: 10.3390/md13106407
关键词
beta-amyloid; Caenorhabditis elegans; cultivated Chondrus crispus; glycolipid; monogalactosyl diacylglycerol (MGDG); neuroprotection; red seaweeds
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)-IRDF
- Atlantic Canada Opportunities Agency (ACOA
- NSERC
- Nova Scotia Department of Agriculture and Marketing (NSDAM)
- Acadian Seaplants Limited
We report here the protective effects of a methanol extract from a cultivated strain of the red seaweed, Chondrus crispus, against -amyloid-induced toxicity, in a transgenic Caenorhabditis elegans, expressing human A(1-42) gene. The methanol extract of C. crispus (CCE), delayed -amyloid-induced paralysis, whereas the water extract (CCW) was not effective. The CCE treatment did not affect the transcript abundance of amy1; however, Western blot analysis revealed a significant decrease of A species, as compared to untreated worms. The transcript abundance of stress response genes; sod3, hsp16.2 and skn1 increased in CCE-treated worms. Bioassay guided fractionation of the CCE yielded a fraction enriched in monogalactosyl diacylglycerols (MGDG) that significantly delayed the onset of -amyloid-induced paralysis. Taken together, these results suggested that the cultivated strain of C. crispus, whilst providing dietary nutritional value, may also have significant protective effects against -amyloid-induced toxicity in C. elegans, partly through reduced -amyloid species, up-regulation of stress induced genes and reduced accumulation of reactive oxygen species (ROS).
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