期刊
MALARIA JOURNAL
卷 14, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12936-015-0731-2
关键词
Plasmodium; Malaria; Low-density; Asymptomatic; Loop-mediated isothermal amplification; Mass screening; DNA contamination
资金
- Global Fund [ZAN-809-G07-M]
- Swedish Medical Research Council (VR) [2009-3785, 2013-6594]
- Foundation for Innovative New Diagnostics (FIND)
- German Federal Ministry of Education and Research (BMBF) through KfW Entwicklungsbank
- Einhorn foundation
- Medical Research Council [MR/K012126/1] Funding Source: researchfish
Background: Molecular tools for detection of low-density asymptomatic Plasmodium infections are needed in malaria elimination efforts. This study reports results from the hitherto largest implementation of loop-mediated isothermal amplification (LAMP) for centralized mass screening of asymptomatic malaria in Zanzibar. Methods: Healthy individuals present and willing to participate in randomly selected households in 60 villages throughout Zanzibar were screened for malaria by rapid diagnostic tests (RDT). In 50% of the study households, participants were asked to provide 60 mu L of finger-prick blood for additional LAMP screening. LAMP was conducted in two centralized laboratories in Zanzibar, by trained technicians with limited or no previous experience of molecular methods. The LAMP assay was performed with Loopamp(TM) MALARIA Pan/Pf Detection Kit (Eiken Chemical Company, Japan). Samples positive for Plasmodium genus (Pan)-LAMP were re-tested using Plasmodium falciparum-specific LAMP kits. Results: Paired RDT and LAMP samples were available from 3983 individuals. The prevalence of asymptomatic malaria was 0.5% (CI 95% 0.1-0.8) and 1.6% (CI 95% 1.1-2.2) by RDT and Pan-LAMP, respectively. LAMP detected 3.4 (CI 95% 2.2-5.2) times more Plasmodium positive samples than RDT. DNA contamination was experienced, but solved by repetitive decontamination of all equipment and reagents. Conclusions: LAMP is a simple and sensitive molecular tool, and has potential in active surveillance and mass-screening programmes for detection of low-density asymptomatic malaria in pre-elimination settings. However, in order to deploy LAMP more effectively in field settings, protocols may need to be adapted for processing larger numbers of samples. A higher throughput, affordable closed system would be ideal to avoid contamination.
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