期刊
MACROMOLECULAR RAPID COMMUNICATIONS
卷 36, 期 21, 页码 1929-1934出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.201500289
关键词
ionomycin; membrane thickness; polymersomes; selective membrane permeability
资金
- Swiss National Science Foundation [200020_140302/1]
- National Center of Competence in Research Molecular Systems Engineering [51NF4O_141825]
- Swiss National Science Foundation (SNF) [200020_140302] Funding Source: Swiss National Science Foundation (SNF)
Biomimetic polymer nanocompartments (polymersomes) with preserved architecture and ion-selective membrane permeability represent cutting-edge mimics of cellular compartmentalization. Here it is studied whether the membrane thickness affects the functionality of ionophores in respect to the transport of Ca2+ ions in synthetic membranes of polymersomes, which are up to 2.6 times thicker than lipid membranes (5 nm). Selective permeability toward calcium ions is achieved by proper insertion of ionomycin, and demonstrated by using specific fluorescence markers encapsulated in their inner cavities. Preservation of polymersome architecture is shown by a combination of light scattering, transmission electron microscopy, and fluorescence spectroscopy. By using a combination of stopped-flow and fluorescence spectroscopy, it is shown that ionomycin can function and transport calcium ions across polymer membranes with thicknesses in the range 10.7-13.4 nm (7.1-8.9 times larger than the size of the ionophore). Thicker membranes induce a decrease in transport, but do not block it due to the intrinsic flexibility of these synthetic membranes. The design of ion selective biomimetic nanocompartments represents a new path toward the development of cellular ion nanosensors and nanoreactors, in which calcium sensitive biomacromolecules can be triggered for specific biological functions.
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