期刊
MACROMOLECULAR MATERIALS AND ENGINEERING
卷 300, 期 8, 页码 802-809出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mame.201500038
关键词
anti-thrombogenic; cell adhesion; PCU; surface modification; zwitterionic polymer
资金
- Ministry of Science and Technology of China [2013DFG52040]
- National Natural Science Foundation of China [31370969]
- Ph.D. Programs Foundation of Ministry of Education of China [20120032110073]
- Program of Introducing Talents of Discipline to Universities of China [B06006]
Herein, we grafted the zwitterionic polynorbornene onto polycarbonate urethane (PCU) film surface by a convenient route of thiol-ene click-chemistry. The PCU film surface was first treated with hexamethylene-1,6-diisocynate and subsequently with two different thiol agents (L-cysteine and beta-marcaptoethanol) in the presence of di-n-butyltin dilaurate (DBTDL) to immobilize sulfhydryl groups onto the surface. Here, DBTDL acted as selective catalyst for the reaction between surface-tethered isocyanates and amine/hydroxyl groups in thiol agents over that of free thiol groups. In the next step, zwitterionic polynorbornene (poly(NSulfoZI)) having functionalizable double bonds was grafted onto these surfaces by photo-initiated thiol-ene click-reaction. The modified surfaces were characterized by water contact angle and XPS analysis. Moreover, the cytocompatibility of these surfaces was investigated by model endothelial cells, EA.hy926, for 1, 3, and 7 d culture times, which showed enhanced cell adhesion and growth. Therefore, the poly(NSulfoZI) functionalized PCU surface using l-cysteine as thiol agent could be a good candidate for tissue engineering material application.
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