期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 60, 期 1, 页码 246-252出版社
ELSEVIER
DOI: 10.1016/j.lwt.2014.05.063
关键词
Esterase; Tributyrin; Halophilic; Lactic acid bacteria; Food fermentation
资金
- MINECO [AGL2011-22745, BFU2010-17929]
- (ALIBIRD) (Comunidad de Madrid) [S2009/AGR-1469]
- INIA [RM2012-00004]
- CSIC
Lactobacillus plantarum is a lactic acid bacteria found in fermented foods on which high salt concentrations are present. So far, no salt-tolerant lipase has been described from lactic acid bacteria. In the present study, the gene lp_3562 encoding a putative esterase/lipase was cloned and expressed in Escherichia coli BL21 (DE3) and the overproduced Lp_3562 protein has been biochemically characterized. Lp_3562 is a lipase active on tributyrin and also on other long chain substrates. Although the highest activity was observed at pH 7, the enzyme showed activity at pH 5.0-8.0. Lp_3562 exhibited optimal lipase activity at 40 degrees C and, showing more than 40% of maximal activity at refrigeration temperature (5 degrees C). Conversely, Lp_3562 presented good thermostability exhibiting more than 80% of its maximal activity after 20 h incubation at 45 degrees C. More interestingly, Lp_3562 was active under NaCl concentrations higher than 20%. The biochemical properties exhibited by Lp_3562 make this halotolerant lipase attractive for its use in food fermentations. (C) 2014 Elsevier Ltd. All rights reserved.
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