期刊
HUMAN REPRODUCTION
卷 27, 期 7, 页码 1939-1945出版社
OXFORD UNIV PRESS
DOI: 10.1093/humrep/des128
关键词
AZFc Y deletion; meiosis; apoptosis; caspase; DNA fragmentation
资金
- Assistance publique hopitaux de Marseille
- Inserm
AZFc deletions of the Y chromosome are the major known genetic cause of spermatogenetic failure. Meiotic studies have shown a prevalence of synaptonemal complex fragmentation and an excess of early-stage sperm cells, suggesting that the maturation block could involve apoptosis. We present a prospective and observational study of apoptotic markers in the sperm of four AZFc-deleted patients and two non-obstructive azoospermic controls without an AZFc deletion. Polycaspases assays and terminal deoxynucleotidyl transferase dUDP nick-end labelling (TUNEL) assays were combined to evaluate the incidence of apoptosis in pre-meiotic, meiotic and post-meiotic germs cells identified, respectively, using anti-melanoma-associated antigen A4 (MAGE-A4), anti-synaptonemal complex protein 3 (SCP3) and anti-sperm acrosome membrane-associated protein 1 (SPACA1) antibodies. We detected apoptosis at all stages of AZFc-deletion spermatogenesis. Using the caspase assay, the incidence of positive cells was found to be heterogeneous for pre-meiotic (from 4.8 to 84.5) and meiotic stages (from 7.9 to 57.6), while for post-meiotic cells, the mean incidence was 6 in AZFc-deleted patients compared with 26.5 in controls (P 0.05). Using the TUNEL assay, the mean percentage with DNA fragmentation for meiotic cells was 54.0 in AZFc-deleted patients compared with 20.3 in controls (P 0.05), while the percentage of TUNEL-positive post-meiotic cells ranged from 5.3 to 44.7. Spermatocyte loss in AZFc-deleted patients occurs via the apoptotic pathway. In post-meiotic cells, the lower incidence of apoptosis in testis from three of the four AZFc-deleted patients, compared with controls, is consistent with AZFc deletions having little negative impact on sperm quality.
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