期刊
HUMAN REPRODUCTION
卷 24, 期 3, 页码 670-678出版社
OXFORD UNIV PRESS
DOI: 10.1093/humrep/den410
关键词
gonocyte; oogonia; ionizing radiation; apoptosis; p53
资金
- INSERM
- Commissariat a l'Energie Atomique (CEA)
- Universite Paris 7-Denis Diderot and Electricite de France (EDF)
- Ministere de l'Education Nationale, de la Recherche et de la Technologie
We have previously shown that male human fetal germ cells are highly radiosensitive and that their death depends on p53 activation. Male germ cell apoptosis was initiated with doses as low as 0.1 Gy and was prevented by pifithrin alpha, a p53 inhibitor. In this study, we investigated the radiosensitivity of early female and male fetal proliferating germ cells. Both male and female fetal germ cells displayed a similar number of gamma H2AX foci in response to ionizing radiation (IR). In organ culture of human fetal ovaries, the germ cells underwent apoptosis only when exposed to high doses of IR (1.5 Gy and above). Accumulation of p53 was detected in irradiated male human fetal germ cells but not in female ones. Inhibition of p53 with pifithrin alpha did not affect oogonia apoptosis following irradiation. IR induced apoptosis similarly in mouse fetal ovaries in organ culture and in vivo during oogonial proliferation. Germ cell survival in testes from p53 knockout or p63 knockout mice exposed to IR was better than wild-type, whereas female germ cell survival was unaffected by p53 or p63 knockout. These findings show that pre-meiotic male and female fetal germ cells behave differently in response to a genotoxic stress-025EFirradiation-025EFwith oogonia being less sensitive and undergoing p53-independent apoptosis.
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