期刊
HUMAN PATHOLOGY
卷 41, 期 12, 页码 1742-1748出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.humpath.2010.06.001
关键词
Gastric cancer; Ribonucleotide reductase M2 subunit; Immunohistochemistry; Epstein-Barr virus; Small interfering RNA
类别
资金
- Japan Society for the Promotion of Science [20249022]
- Grants-in-Aid for Scientific Research [22890037] Funding Source: KAKEN
Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, which are required for DNA synthesis This study was conducted to investigate the roles of ribonucleotide reductase M2 subunit in gastric cancer The expression of ribonucleotide reductase M2 subunit protein was examined by immunohistochemistry In normal gastric mucosa, ribonucleotide reductase M2 subunit expression was restricted to the neck regions of gastric pits and no expression was observed in the surface epithelium Among 112 gastric cancer tissues, ribonucleotide reductase M2 subunit overexpression (>= 10% cancer cells stained) was observed in 72 cases (64 3%) Ribonucleotide reductase M2 subunit overexpression was significantly associated with male sex (P = 015), presence of muscularis propria invasion (P = 020), presence of Epstein-Barr virus (P = 045), expression of survivin (P = 0014), and DNA methyltransferase I (P = 043), but not with age, histology, tumor size, lymph node metastasis or expression of phosphatase and tensin homolog, phosphorylated signal transducer, and activator of transcription 3 or p53 Suppression of ribonucleotide reductase M2 subunit synthesis, using small interfering RNA, inhibited the growth of 3 gastric cancer cell lines, MKN-1, MKN-7, and SNU-719 Our data suggest that ribonucleotide reductase M2 subunit overexpression could be associated with the gastric cancer progression and that suppression of its function is a potential therapeutic strategy in gastric cancer (C) 2010 Elsevier Inc All rights reserved
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据