期刊
HUMAN MUTATION
卷 30, 期 7, 页码 1054-1061出版社
WILEY
DOI: 10.1002/humu.21007
关键词
Parkinson disease; PARK2; LRRK2; SNCA; CNV
资金
- University of Antwerp
- Fund for Scientific Research Flanders (FWO-F)
- Institute for Science and Technology-Flanders (IWT-F)
- Foundation for Alzheimer Research (SAO/FRMA)
- Medical Research Foundation Antwerp and Neurosearch Antwerp
- Belgian Science Policy Office [P6/43]
The relative contribution of simple mutations and copy number variations (CNVs) in SNCA, PARK2, PINK1, PARK7, and LRRK2 to the genetic etiology of Parkinson disease (PD) is still unclear because most studies did not completely analyze each gene. In a large group of Belgian PD patients (N=310) and control individuals (N=270), we determined the mutation frequency of both simple mutations and CNVs in these five PD genes, using direct sequencing, multiplex amplicon quantification (MAQ), and real-time PCR assays. Overall, we identified 14 novel heterozygous variants, of which I I were absent in control individuals. We observed eight PARK2 (multiple) exon multiplications in PD patients and one exon deletion in a control individual. Furthermore, we identified one SNCA whole,gene duplication. The PARK2 and LRRK2 mutation frequencies in Belgian PD patients were similar to those reported in other studies. However, at this stage the true pathogenic nature of some heterozygous mutations in recessive genes remains elusive. Furthermore, though mutations is SNCA, PINK1, and PARK7 are rare, our identification of a SNCA duplication confirmed that screening of these genes remains meaningful. Hum Mutat 30:1054-1061, 2009. (C) 2009 Wiley-Liss, Inc.
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