期刊
LASERS IN MEDICAL SCIENCE
卷 30, 期 3, 页码 1153-1159出版社
SPRINGER LONDON LTD
DOI: 10.1007/s10103-015-1722-9
关键词
Methicillin-resistant Staphylococcus aureus; Hyperbaric oxygen therapy; Antimicrobial activity; Phototherapy; Low-level light therapy; Blue light
资金
- Clinical and Translational Science Award (CTSA) program of the National Center for Research Resources
- National Center for Advancing Translational Sciences [UL1RR031973]
- College of Health Sciences, University of Wisconsin, Milwaukee, USA
It has been shown that, in vitro, hyperbaric oxygen (HBO) suppresses 28 % bacterial growth, while 470-nm blue light alone suppresses up to 92 % methicillin-resistant Staphylococcus aureus (MRSA) in one application in vitro. Therefore, we determined if combined 470-nm light (55 J/cm(2)) and HBO will yield 100 % bacterial suppression in experimental simulation of mild, moderate or severe MRSA infection. We cultured MRSA at 3 x 10(6), 5 x 10(6), 7 x 10(6), 8 x 10(6), or 12 x 10(6) CFU/ml and treated each concentration in four groups as follows: (1) control (no treatment) (2) photo-irradiation only, (3) photo-irradiation then HBO, (4) HBO only, and (5) HBO then photo-irradiation. Bacteria colonies were then quantified. The results showed that at each bacterial concentration, HBO alone was significantly less effective in suppressing MRSA than photo-irradiation or combined HBO and photo-irradiation (p < 0.0001). Similarly, at no bacterial concentration did combined HBO and 470-nm light treatment yield a statistically better result than 470-nm light alone (p > 0.05), neither did HBO treatment either before or after irradiation make a difference. Furthermore, at no bacterial concentration was 100 % MRSA suppression achieved. Indeed, the maximum bacterial suppression attained was in the mild infection model (3 x 10(6) CFU/ml), with blue light producing 97.3 +/- 0.2 % suppression and HBO + 55 J/cm(2) yielding 97.5 +/- 2.5 % suppression. We conclude that (1) HBO and 470-nm light individually suppress MRSA growth; (2) 470-nm blue light is more effective in suppressing MRSA than HBO; and (3) HBO did not act synergistically to heighten the bactericidal effect of 470-nm light.
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