期刊
LASERS IN MEDICAL SCIENCE
卷 31, 期 2, 页码 297-304出版社
SPRINGER LONDON LTD
DOI: 10.1007/s10103-015-1859-6
关键词
Antibiotic-resistant bacteria; Biofilms; Photodynamic inactivation; HMME
资金
- National Natural Science Foundation of China [81401710]
- China Post-doctoral Science Foundation [2014M562424]
- Fundamental Research Funds for the Central Universities
The worldwide increase in bacterial antibiotic resistance has led to a search for alternative antibacterial therapies. A promising approach to killing antibiotic-resistant bacteria is photodynamic antimicrobial chemotherapy, which uses light in combination with a photosensitizer to induce a phototoxic reaction. We evaluated the photodynamic inactivation (PDI) efficiency of hematoporphyrin monomethyl ether (HMME) on antibiotic-resistant bacteria and biofilms. HMME exhibited no significant dark toxicity and provided dose-dependent inactivation of antibiotic-resistant bacteria and biofilms. After incubation with 100-mu M HMME and irradiation with 72-J cm(-2) white light, 4.19-7.59 log(10) reductions in survival were achieved in planktonic suspension. Antibiotic-resistant strains were as susceptible to PDI in biofilms as in planktonic suspensions, but the inactivation of bacterial cells in biofilms was attenuated. In addition, gram-positive bacterial strains and biofilms were more susceptible than gram-negative strains and biofilms to the PDI effect of HMME. Thus, HMME is a promising photosensitizer for the treatment of infectious diseases caused by antibiotic-resistant bacteria, especially gram-positive bacteria.
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