期刊
HUMAN IMMUNOLOGY
卷 70, 期 7, 页码 496-501出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2009.04.001
关键词
Luminex (R) assay; Antibody testing; Nonspecific reactivity; Crossmatch testing
类别
Substances occurring naturally in the sera of patients can interfere with Luminex (R) antibody assays, causing increased background and changes in antibody specificity. We present data on the effectiveness of hypotonic dialysis (HD) or dithiothreitol (DTT) treatment in eliminating this interference. HD significantly increased reaction strength of positive control beads and reduced reaction strength of negative control beads. HID also improved specificity identification, determination of donor-specific antibody (DSA) strength, and crossmatch predictability compared with values in untreated serum. DTT also increased the reaction strength of positive control beads, but in most cases, further increased reactivity of negative control beads. DTT improved crossmatch predictability but to a lesser extent than did HD and may differ with specificities defined in other assays. Because interference is frequently observed in sera from highly sensitized patients, it is important to recognize and eliminate interference in Luminex (R) antibody assays for accurate and meaningful test interpretation. (C) 2009 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据