期刊
HUMAN IMMUNOLOGY
卷 70, 期 12, 页码 1010-1015出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2009.07.030
关键词
gamma-Radiation; HLA-G; Melanoma; Immune system; Immunotolerance
类别
资金
- Commissariat a l'Energie Atomique
- Nuclear Regulatory Authority-Argentina
- MINCYT-ECOS (Argentina-France) Scientific-Technological Cooperation [A07SO2]
Human leukocyte antigen G (HLA-G) is a nonclassical HL-A class I molecule involved in fetus protection from the maternal immune system, transplant tolerance, and viral and tumoral immune escape. Tumor-specific HLA-G expression has been described for a wide variety of malignancies, including melanomas. The aim of this study was to evaluate whether ionizing radiation (IR) could modulate the surface expression of HLA-G1 in a human melanoma cell line that expresses endogenously membrane-bound HLA-G1. For this purpose, cells were exposed to increasing doses of gamma-irradiation (0-20 Gy) and HLA-G1 levels at the plasma membrane were analyzed at different times postirradiation by flow cytometry. HLA-G total expression and the presence of the Soluble form of HLA-G1 (sHLA-G1) in the culture medium of irradiated cells were also evaluated. IR was capable of downregulating cell surface and total HLA-G levels, with a concomitant increase of sHLA-G1 in the medium. These results could indicate that gamma-irradiation decreases HLA-G1 surface levels by enhancing the proteolytic cleavage of this molecule. (C) 2009 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
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