期刊
HISTOCHEMISTRY AND CELL BIOLOGY
卷 141, 期 6, 页码 629-638出版社
SPRINGER
DOI: 10.1007/s00418-014-1192-3
关键词
Single-molecule localization microscopy; Fluorescence microscopy; Resolution; Localization precision; Single-molecule fluorescence
资金
- German Ministry of Education and Research [0315262, 0316170D]
- cluster of excellence Macromolecular Complexes (CEF, DFG cluster of excellence) [EXC 115]
The localization precision is a crucial and important parameter for single-molecule localization microscopy (SMLM) and directly influences the achievable spatial resolution. It primarily depends on experimental imaging conditions and the registration potency of the algorithm used. We propose a new and simple routine to estimate the average experimental localization precision in SMLM, based on the nearest neighbor analysis. By exploring different experimental and simulated targets, we show that this approach can be generally used for any 2D or 3D SMLM data and that reliable values for the localization precision sigma (SMLM) are obtained. Knowing sigma (SMLM) is a prerequisite for consistent visualization or any quantitative structural analysis, e.g., cluster analysis or colocalization studies.
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