期刊
HISTOCHEMISTRY AND CELL BIOLOGY
卷 141, 期 4, 页码 365-381出版社
SPRINGER
DOI: 10.1007/s00418-013-1167-9
关键词
Nucleolus; AFM; Stiffness; Nucleolar structure
资金
- Japanese Society for the Promotion of Sciences (JSPS)
- MEXT Japan [24115512, 24115003]
- Grants-in-Aid for Scientific Research [24115512, 24115003] Funding Source: KAKEN
In eukaryotic cells, ribosome biogenesis occurs in the nucleolus, a membraneless nuclear compartment. Noticeably, the nucleolus is also involved in several nuclear functions, such as cell cycle regulation, non-ribosomal ribonucleoprotein complex assembly, aggresome formation and some virus assembly. The most intriguing question about the nucleolus is how such dynamics processes can occur in such a compact compartment. We hypothesized that its structure may be rather flexible. To investigate this, we used atomic force microscopy (AFM) on isolated nucleoli. Surface topography imaging revealed the beaded structure of the nucleolar surface. With the AFM's ability to measure forces, we were able to determine the stiffness of isolated nucleoli. We could establish that the nucleolar stiffness varies upon drastic morphological changes induced by transcription and proteasome inhibition. Furthermore, upon ribosomal proteins and LaminB1 knockdowns, the nucleolar stiffness was increased. This led us to propose a model where the nucleolus has steady-state stiffness dependent on ribosome biogenesis activity and requires LaminB1 for its flexibility.
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