期刊
HISTOCHEMISTRY AND CELL BIOLOGY
卷 132, 期 4, 页码 383-394出版社
SPRINGER
DOI: 10.1007/s00418-009-0624-y
关键词
Microgravity; Skeletal muscle atrophy; SERCA; Bed rest; Countermeasures; Women's international space exploration (WISE) 2005
资金
- European Space Agency (ESA) [14431/02/NL/SH2]
- BMBF/German AeroSpace (DLR) [50WB0521, 50WB0821]
We monitored changes in SERCA isoform specific expression and S-nitrosylation in myofibers of lower limb soleus (SOL) and vastus lateralis (VL) muscle biopsies before and after 60 days of voluntary long term bed rest (BR) without (BR-CTRL group, n = 8) and with exercise countermeasure (BR-EX group, n = 8). Before BR, a typical myofiber type-specific distribution of fast and slow SERCA1/2a isoforms was seen. After BR, a subpopulation (approx. 15%) of slow myofibers in BR-CTRL additionally expressed the fast SERCA1a isoform which was not seen in BR-EX. After BR, SERCA1a S-nitrosylation patterns analyzed by the biotin-switch assay decreased in disused SOL only but increased in both muscles following exercise. Differential SERCA1a S-nitrosylation and SERCA1a/2a co-expression in subsets of slow myofibers should be considered as signs of an altered cytosolic Ca2+ homeostasis following chronic muscle disuse. Exercise preserved myofiber type-specific SERCA1a expression and S-nitrosylation in VL and SOL in a different way, suggesting muscle-specific responses to the countermeasure protocol applied during bed rest.
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