期刊
HIPPOCAMPUS
卷 24, 期 11, 页码 1404-1412出版社
WILEY
DOI: 10.1002/hipo.22322
关键词
cognition; dentate gyrus; Zn2+ signaling; zinc chelator; ZnAF-2DA; clioquinol
资金
- Grants-in-Aid for Scientific Research [26460172] Funding Source: KAKEN
The role of perforant pathway-dentate granule cell synapses in cognitive behavior was examined focusing on synaptic Zn2+ signaling in the dentate gyrus. Object recognition memory was transiently impaired when extracellular Zn2+ levels were decreased by injection of clioquinol and N,N,N,N-tetrakis-(2-pyridylmethyl) ethylendediamine. To pursue the effect of the loss and/or blockade of Zn2+ signaling in dentate granule cells, ZnAF-2DA (100 pmol, 0.1 mM/1 mu l), an intracellular Zn2+ chelator, was locally injected into the dentate molecular layer of rats. ZnAF-2DA injection, which was estimated to chelate intracellular Zn2+ signaling only in the dentate gyrus, affected object recognition memory 1 h after training without affecting intracellular Ca2+ signaling in the dentate molecular layer. In vivo dentate gyrus long-term potentiation (LTP) was affected under the local perfusion of the recording region (the dentate granule cell layer) with 0.1 mM ZnAF-2DA, but not with 1-10 mM CaEDTA, an extracellular Zn2+ chelator, suggesting that the blockade of intracellular Zn2+ signaling in dentate granule cells affects dentate gyrus LTP. The present study demonstrates that intracellular Zn2+ signaling in the dentate gyrus is required for object recognition memory, probably via dentate gyrus LTP expression. (c) 2014 Wiley Periodicals, Inc.
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