期刊
HIPPOCAMPUS
卷 20, 期 4, 页码 513-523出版社
WILEY-LISS
DOI: 10.1002/hipo.20647
关键词
cell proliferation; antidepressant; running; 2-AG; FAAH; neuroprotection
资金
- NIH [R21DA022439]
- MSFHR
- NSERC
- Pacifica Family Addictions Foundation
- Pacifica Century Graduate Scholarship
- Portugese FCT
- CIHR
- Research for a Healthier Tomorrow
- Medical College of Wisconsin
- NATIONAL INSTITUTE ON DRUG ABUSE [R21DA022439] Funding Source: NIH RePORTER
Voluntary exercise and endogenous cannabinoid activity have independently been shown to regulate hippocampal plasticity. The aim of the current study was to determine whether the endocannabinoid system is regulated by voluntary exercise and if these changes contribute to exercise-induced enhancement of cell proliferation. In Experiment 1, 8 days of free access to a running wheel increased the agonist binding site density of the cannabinoid CB(1) receptor; CB(1) receptor-mediated GTP gamma S binding; and the tissue content of the endocannabinoid anandamide in the hippocampus but not in the prefrontal cortex. In Experiment 2, the CB, receptor antagonist AM251 (1 mg kg(-1)) was administered daily to animals given free access to a running wheel for 8 days, after which cell proliferation in the hippocampus was examined through immunohistochemical analysis of the cell cycle protein Ki-67. Voluntary exercise increased proliferation of progenitor cells, as evidenced by the increase in the number of Ki-67 positive cells in the granule cell layer of the dentate gyrus (DG) in the hippocampus. However, this effect was abrogated by concurrent treatment with AM251, indicating that the increase in endocannabinoid signaling in the hippocampus is required for the exercise-induced increase in cell proliferation. These data demonstrate that the endocannabinoid system in the hippocampus is sensitive to environmental change and suggest that it is a mediator of experience-induced plasticity. (C) 2009 Wiley-Liss, Inc.
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