期刊
HIGH PRESSURE RESEARCH
卷 33, 期 1, 页码 64-72出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08957959.2013.767898
关键词
high hydrostatic pressure; compression rate; decompression rate; E; coli
The effect of compression and decompression rates of high hydrostatic pressure (HHP) on Escherichia coli O157:H7 was investigated. Samples of orange juice, skimmed milk and Tris buffer were inoculated with E. coli O157:H7 and subjected to 600MPa for 3min at 4 degrees C with fast, medium and slow compression and decompression. Analyses immediately after HHP treatment revealed that E. coli in milk and juice treated with fast compression suffered more than slow compression rates. Slow decompression resulted in higher inactivation of E. coli in all matrices. After overnight storage, highest stress-recovery (1.19logcfu/mL) was observed in Tris buffer. Healthy cells were<1logcfu/mL in milk and buffer samples, but no growth was detected in orange juice for any of the treatments immediately after HHP. After 15 days at 4 degrees C, E. coli cells in skimmed milk and Tris buffer recovered significantly, whereas the recovery of sublethally injured cells was inhibited in orange juice.
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