4.5 Article

Intraspecific variation in expression of candidate genes for osmoregulation, heme biosynthesis and stress resistance suggests local adaptation in European flounder (Platichthys flesus)

期刊

HEREDITY
卷 101, 期 3, 页码 247-259

出版社

NATURE PUBLISHING GROUP
DOI: 10.1038/hdy.2008.54

关键词

gene expression; quantitative PCR; local adaptation; genetic population structure; microarray

资金

  1. SLIP research school
  2. Danish Ministry for Food, Agriculture and Fisheries
  3. Danish Agricultural and Veterinary Research Council
  4. Danish Institute for Fisheries Research
  5. Elisabeth and Knud Petersens Foundation
  6. Natural Environment Research Council [NE/C50767X/1, NE/C507710/1, NE/C507702/1, NE/C507688/1, NE/C507661/1] Funding Source: researchfish

向作者/读者索取更多资源

Despite the recent discovery of significant genetic structuring in a large number of marine organisms, the evolutionary significance of these often minute genetic differences are still poorly understood. To elucidate the adaptive relevance of low genetic differentiation among marine fish populations, we studied expression differences of osmoregulatory and stress genes in genetically weakly differentiated populations of the European flounder (Platichthys flesus), distributed across a natural salinity gradient. Flounders were maintained in a long-term reciprocal transplantation experiment mimicking natural salinities in the North Sea and the Baltic Sea. Applying real-time quantitative PCR and microarray analysis we studied expression of four candidate genes (hsp70, angiotensinogen, Na/K-ATPase-alpha and 5-aminolevulinic acid synthase (ALAS)) in gill, kidney and liver tissues. Genes involved in osmoregulative processes (Na/K-ATPases-alpha and angiotensinogen) showed highly plastic but similar expression in the two populations dependent on environmental salinity. However, we observed a unique sixfold up-regulation of hsp70 in kidney tissue of flounder from the North Sea following long-term acclimation to Baltic salinities. Similarly, significant differences between North Sea and Baltic flounders in expression of ALAS in response to different salinities were found in gill and liver tissue. These findings strongly suggest that gene expression in flounders is shaped by adaptation to local environmental conditions. This identification of adaptive differences in high gene flow marine organisms adds a new dimension to our current understanding of evolutionary processes in the sea and is of paramount importance for identification, protection and sustainable management of marine biodiversity.

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