期刊
HEPATOLOGY
卷 56, 期 5, 页码 1861-1869出版社
WILEY
DOI: 10.1002/hep.25854
关键词
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资金
- Spanish Ministry of Health, Instituto de Salud Carlos III (Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas (CIBERehd)) [PS09/00485, PI051871]
- Comunidad de Madrid [MITIC-CM S-BIO-0189/2006]
- Spanish Ministry of Health, Instituto de Salud Carlos III [PI08/1890]
- Instituto de Salud Carlos III
- Spanish Ministry of Education [BES-2008-003209]
Cirrhosis with ascites is associated with a high rate of gut bacterial translocation (GBT) and spontaneous bacterial infections of enteric origin. We addressed the activation state and role of intestinal dendritic cells (DCs) in experimental ascitic cirrhosis and their relationship with GBT. Cirrhosis with ascites was CCl4 induced in rats. To examine their activation state and functions, DCs (CD103(+)RT1B(+)CD3(-)CD45RA(-)) were isolated from the intestinal lamina propria and mesenteric lymph nodes (MLNs), and the following parameters were determined by flow cytometry: surface antigen expression; spontaneous or lipopolysaccharide-stimulated tumor necrosis factor alpha (TNF-alpha) production; and in vitro capacity to phagocytose latex beads and to migrate toward the chemokine (C-C motif) ligand 21. GBT was defined as the growth of bacteria in MLNs culture. Bacterial DNA (Bact-DNA) in MLNs was identified by polymerase chain reaction. In rats with Bact-DNA in MLNs without GBT, intestinal and MLNs CD103(+)-DCs showed features of activation, expansion of the proinflammatory CD4(+)-DC subpopulation, augmented TNF-alpha production, and increased phagocytic and migratory capacities. In contrast, in rats with GBT, CD103(+)-DCs showed the absence of an activated phenotype, lowered TNF-alpha production, and relatively deficient phagocytosis and migration capacities. The CD103(+)-DC of rats without Bact-DNA in MLNs or GBT were similar to controls. In cirrhotic rats, bowel decontamination with antibiotics eliminated Bact-DNA in MLNs and GBT, normalized the activation state and functions of CD103(+)-DCs, and increased their TNF-alpha production. Conclusion: In experimental cirrhosis with ascites, continuous pressure of gut bacteria shapes the phenotypic and functional profile of intestinal DCs to produce effects that range from their activation and enhanced functions to their exhaustion and tolerance. (HEPATOLOGY 2012;56:18611869)
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