期刊
HEPATOLOGY
卷 54, 期 4, 页码 1293-1302出版社
WILEY-BLACKWELL
DOI: 10.1002/hep.24526
关键词
-
资金
- National Institutes of Health (Bethesda, MD) [DK39588]
- Grants-in-Aid for Scientific Research [21590433] Funding Source: KAKEN
In primary biliary cirrhosis (PBC), patients develop a multilineage response to a highly restricted peptide of the E2 component of pyruvate dehydrogenase (PDC-E2) involving autoantibody and autoreactive cluster of differentiation (CD)4(+) and CD8(+) T-cell responses. Recent data from murine models have suggested that liver-infiltrating CD8(+) cells play a critical role in biliary destruction in PBC. We hypothesized that chronic antigen stimulation of CD8(+) T cells alters effector memory T cell (T(EM)) frequency and function similar to that seen with chronic viral infections, including failure to terminally differentiate and relative resistance to apoptosis. We have rigorously phenotyped CD8(+) T-cell subpopulations from 132 subjects, including 76 patients with PBC and 56 controls, and report a higher frequency of T(EM) cells characterized as CD45RO(high)CD57(+)CD8(high), but expressing the gut homing integrin, alpha 4 beta 7, in peripheral blood mononuclear cells of PBC. These CD8(high) T(EM) cells have reduced expression of Annexin V after TCR stimulation. Consistent with a T(EM) phenotype, CD45RO(high)CD57(+)CD8(high) T cells express higher levels of granzyme A, granzyme B, perforin, CCR5 and alpha 4 beta 7, and lower levels of CCR7 and CD28 than other CD8(high) T cells. Furthermore, interleukin (IL)-5 produced by CD8(+) CD57(+) T lymphocytes upon in vitro T-cell receptor stimulation are increased in PBC. Histologically, CD8(+)CD57(+) T cells accumulate around the portal area in PBC. Moreover, CD8(+)CD57(+) T cells respond specifically to the major histocompatibility class I epitope of PDC-E2. Conclusion: In conclusion, our data demonstrate that CD45RO(high)CD57(+)CD8(high) T cells are a subset of terminally differentiated cytotoxic TEM cells, which could play a critical role in the progressive destruction of biliary epithelial cells. (HEPATOLOGY 2011;54:1293-1302)
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