期刊
HEART RHYTHM
卷 10, 期 3, 页码 412-419出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.hrthm.2012.11.018
关键词
AC; Arrhythmogenic cardiomyopathy; Connexin 43; Na(V)1.5; Desmosome; Plakophilin-2; Plakoglobin; Immunohistochemistry
资金
- Netherlands Heart Foundation [2007B139]
- Interuniversity Cardiology Institute of the Netherlands [06901]
- US National Institutes of Health [HL102361, HL084583, HL083422, HL096805]
- American Heart Association [12SDG8800009]
- National Institutes of Health [RO1-HL106632, PO1-HL087226, RO1-GM67691]
- Foundation Leducq Transatlantic Network
- Saving Tiny Hearts Society
- Fondation Leducq
- American Heart Association
- Gilead Sciences Research Scholars Program
BACKGROUND Arrhythmogenic cardiomyopathy (AC) is closely associated with desmosomal mutations in a majority of patients. Arrhythmogenesis in patients with AC is likely related to remodeling of cardiac gap junctions and increased levels of fibrosis. Recently, using experimental models, we also identified sodium channel dysfunction secondary to desmosomal dysfunction. OBJECTIVE To assess the immunoreactive signal levels of the sodium channel protein Na(V)1.5, as well as connexin43 (Cx43) and plakoglobin (PKG), in myocardial specimens obtained from patients with AC. METHODS left and right ventricular free wall postmortem material was obtained from 5 patients with AC and 5 controls matched for age and sex. Right ventricular septal biopsies were taken from another 15 patients with AC. All patients fulfilled the 2010 revised Task Force Criteria for the diagnosis of AC. Immunohistochemical analyses were performed using antibodies against Cx43, PKG, Na(V)1.5, plakophilin-2, and N-cadherin. RESULTS N-cadherin and desmoplakin immunoreactive signals and distribution were normal in patients with AC compared to controls. Plakophilin-2 signals were unaffected unless a plakophilin-2 mutation predicting haploinsufficiency was present. Distribution was unchanged compared to that in controls. Immunoreactive signal levels of PKG, Cx43, and Na(V)1.5 were disturbed in 74%, 70%, and 65% of the patients, respectively. CONCLUSIONS A reduced immunoreactive signal of PKG, Cx43, and Na(V)1.5 at the intercalated disks can be observed in a large majority of the patients. Decreased levels of Na(V)1.5 might contribute to arrhythmia vulnerability and, in the future, potentially could serve as a new clinically relevant toot for risk assessment strategies.
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