Release kinetics of early ischaemic biomarkers in a clinical model of acute myocardial infarction

Objective To determine the release kinetics of different biomarkers with potential as novel early ischaemic biomarkers in patients with acute coronary syndrome (ACS); it is difficult to establish the detailed release kinetics in patients with acute myocardial infarction (AMI). Methods We analysed the release kinetics of soluble fms-like tyrosine kinase (sFlt-1), ischaemia modified albumin (IMA), and heart-type fatty acid binding protein (hFABP) in patients with hypertrophic obstructive cardiomyopathy who were undergoing transcoronary ablation of septal hypertrophy (TASH), a procedure mimicking AMI. Consecutive patients (n=21) undergoing TASH were included. Blood samples were collected before TASH and 15, 30, 45, 60, 75, 90, and 105min and 2, 4, 8, and 24h after TASH. sFlt-1 and hFABP were quantified in serum, and IMA was quantified in plasma using immunoassays. Results sFLT-1 and hFABP increased significantly 15min after induction of AMI vs baseline as follows: sFlt-1, 3657.5ng/L (IQR 2302.3-4475.0) vs 76.0ng/L (IQR 71.2-88.8) (p<0.001); hFABP, 9.0ng/mL (IQR 7.0-15.4) vs 4.6ng/mL (IQR 3.4-7.1) (p<0.001). sFlt-1 demonstrated a continuous decrease after the 15th min. hFABP showed a continuous increase until the 8th hour with a decline afterwards. The IMA concentrations increased significantly 30min after induction of AMI vs baseline, with values of 26.0U/mL (IQR 21.8-38.6) vs 15.6U/mL (IQR 10.1-24.7) (p=0.02), and then decreased after 75min. Conclusions sFlt-1 and hFABP increased very early after induction of myocardial ischaemia, showing different release kinetics. The additional information provided by these findings is helpful for developing their potential combined use with cardiac troponins in patients with suspected AMI.