期刊
LAB ON A CHIP
卷 15, 期 5, 页码 1276-1283出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4lc01038h
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资金
- University of Basel Research Grant
- Swiss Multiple Sclerosis Society
- Novartis Foundation
- ERC Starting Grant [337986]
- Swiss National Science Foundation
- SNF Systems X Grant NeuroStemX
- Swiss NCCR Molecular Systems Engineering
- European Research Council (ERC) [337986] Funding Source: European Research Council (ERC)
Dynamical analysis of single-cells allows assessment of the extent and role of cell-to-cell variability, however traditional dish-and-pipette techniques have hindered single-cell analysis in quantitative biology. We developed an automated microfluidic cell culture system that generates stable diffusion-based chemokine gradients, where cells can be placed in predetermined positions, monitored via single-cell time-lapse microscopy, and subsequently be retrieved based on their migration speed and directionality for further off-chip gene expression analysis, constituting a powerful platform for multiparameter quantitative studies of single-cell chemotaxis. Using this system we studied CXCL12-directed migration of individual human primary T cells. Spatiotemporally deterministic retrieval of T cell subsets in relation to their migration speed, and subsequent analysis with microfluidic droplet digital-PCR showed that the expression level of CXCR4 - the receptor of CXCL12 - underlies enhanced human T cell chemotaxis.
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