期刊
LAB ON A CHIP
卷 15, 期 14, 页码 2928-2938出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5lc00541h
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资金
- National Health Research Institutes [03-A1 BNMP11-014]
In vitro culture of single cells facilitates biological studies by deconvoluting complications from cell population heterogeneity. However, there is still a lack of simple yet high-throughput methods to perform single cell culture experiments. In this paper, we report the development and application of a microfluidic device with a dual-well (DW) design concept for high-yield single-cell loading (similar to 77%) in large microwells (285 and 485 mu m in diameter) which allowed for cell spreading, proliferation and differentiation. The increased single-cell loading yield is achieved by using sets of small microwells termed capture-wells and big microwells termed culture-wells according to their utilities for single-cell capture and culture, respectively. This novel device architecture allows the size of the culture microwells to be flexibly adjusted without affecting the single-cell loading efficiency making it useful for cell culture applications as demonstrated by our experiments of KT98 mouse neural stem cell differentiation, A549 and MDA-MB-435 cancer cell proliferation, and single-cell colony formation assay with A549 cells in this paper.
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