期刊
HARMFUL ALGAE
卷 10, 期 6, 页码 702-705出版社
ELSEVIER
DOI: 10.1016/j.hal.2011.05.006
关键词
Pinnatoxin G; Dinoflagellate; Peridiniales; Japan; Australasia
资金
- New Zealand Ministry of Science and Innovation [CAW0703]
A thecate dinoflagellate (Peridiniales, Dinophyceae) was cultured from individual non-calcareous cysts, isolated from surface sediment samples collected in Okinawa, Japan. Two isolates (CAWD188 and CAWD190) from two different sampling sites, each cultured from an individual cyst, produced ca. 11.9 and 15 pg cell(-1) pinnatoxin G, respectively, as determined by liquid chromatography-mass spectrometric (LC-MS). No other pinnatoxins were detected. The motile cells and cysts appeared morphologically identical to those of pinnatoxins E and F producers isolated from New Zealand waters and pinnatoxins E, F, and G producers isolated from Australian waters. Motile and cysts cells measured an average of 25 mu m long x 21.3 mu m wide and 29 mu m long x 25.5 mu m wide, respectively. Analysis of the large subunit ribosomal DNA sequence data showed two well supported strains with slight differences between the Japanese and the Australasian isolates. (C) 2011 Elsevier B.V. All rights reserved.
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