Identification of Novel Lectins from Freshwater Prawn, Macrobrachium rosenbergii, and Expression Analysis in Response to Vibrio harveyi and M. rosenbergii nodavirus

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作者

Snigdha Baliarsingh¹ , Bharat Bhusan Patnaik¹

1. Fakir Mohan University

会议/活动

FMU Research Scholar Conclave, October 2021 (Balasore, Odisha, India)

海报摘要

Lectins are PRRs that binds to PAMPs and participate in the innate immunity of the host. We identified and characterized novel lectins from the freshwater prawn, Macrobrachium rosenbergii such as MrCTL and Mr(T)Lec-4 showing C-type lectin domain and MrERGIC-53 showing L-type lectin domain. MrCTL, Mr(T)Lec-4, and MrERGIC-53 encoded polypeptides of 217, 330, and 504 amino acids, respectively. While Mr(T)Lec-4 mRNA upregulated 6h in the hepatopancreas post-infection with Vibrio harveyi, MrCTL and MrERGIC-53 mRNA showed upregulation in stomach and intestine following M. rosenbergii nodavirus (MrNV) challenge. This demonstrates that the novel lectins putatively participate in the innate immunity of the host.

关键词

Macrobrachium rosenbergii, Lectins, Inate immunity, Vibrio harveyi, M. rosenbergii nodavirus, Expression analysis

研究领域

Bioinformatics and Genomics, Genetics, Zoology, Biological Sciences, Molecular and Cellular Biosciences

参考文献

1. Baliarsingh, S., Dash, M., Dewangan, N., Hameed, A.S.S., Mohanty, J., Lee, Y.S., Patnaik, B.B. (2019). Upregulation of hepatopancreas associated lectin transcripts in Macrobrachium rosenbergii in response to Vibrio harveyi and M. rosenbergii nodavirus. Journal of Environmental Biology 40(5), 969-976.
2. Baliarsingh, S., Sahoo, S., Jo, Y.H., Han, Y.S., Sarkar, A., Lee, Y.S., Mohanty, J., Patnaik, B.B. (2022). Molecular cloning, sequence characterization, and expression analysis of C-type lectin (CTL) and ER-Golgi intermediate compartment 53-kDa protein (ERGIC-53) homologs from the freshwater prawn, Macrobrachium rosenbergii. Aquaculture International 30, 1011-1035.

基金

1. Department of Biotechnology, Government of India (No. BT/PR12710/AAQ/03/713/2015)

补充材料

1. Photograph of poster presentation at FMU Research Scholar Conclave.   Download

附加信息

利益冲突

None declared.

数据可用性声明

The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.

知识共享许可协议

Copyright © 2022 Baliarsingh et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.