What are the environmental impacts of burning non-hazardous agricultural waste such as wood, straw, and grass in open spaces, and what pollutants are produced during this process?
Dr. Palmer’s research interests and effort support preclinical and clinical studies concerning the major obstacles to disease control/cure of hematologic malignancies and successful hematopoietic stem cell transplantation.
The speaker's Lab is focused on understanding the biology of under-studied subtypes of lung cancer, specifically squamous and small cell lung cancer (SCLC). They investigate mechanisms of tumor initiation, progression, plasticity, and drug resistance to uncover vulnerabilities that can be therapeutically targeted. They capitalize on state-of-the-art mouse and patient-derived models to identify and test novel treatment strategies, with the goal of translating these findings to the clinic.
The authors adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
They used the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. They showed that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
They engineered the type II bacterial CRISPR/Cas system to use custom guide RNA (gRNA) for targeted genome editing in human cells, achieving editing rates of up to 25% in different cell types. This system allows for sequence-specific and multiplexable editing across approximately 40.5% of human exons, establishing a versatile tool for robust human genome engineering.
STAR (Structured Transparent Accessible Reproducible) Protocols is an open-access peer-reviewed protocol journal from Cell Press that publishes step-by-step experimental and computational protocols from all areas of life, health, earth, and physical sciences. The primary criteria for publication in STAR Protocols are usability and reproducibility.
They conducted an electronic database search on Medline through July 2011 to identify, compare and discuss publications concerning the effects of cell-cell stimulation, bioactive factors, biomaterials and combinations thereof in terms of cell isolation, proliferation, differentiation and matrix protein synthesis. This survey and discussion might serve as a source for designing future biological treatment strategies for the human IVD.
They found that M1, a molecule promoting mitochondrial fusion and transport, not only boosts mitochondrial dynamics and axon regeneration in the PNS but also facilitates recovery in the central nervous system (CNS) after optic nerve injury, improving neural activity and visual function. This includes complete recovery of pupillary light reflexes and responses to visual threats, highlighting the critical role of mitochondrial dynamics in neural repair.
Current ISC culture systems face challenges such as undefined matrix compositions and variability, complicating research and clinical applications. We developed a defined, collagen-based BLT Sandwich culture system that enhances the growth and expansion of Lgr5+ intestinal epithelial cells, outperforming conventional Matrigel cultures and showing potential for advancing ISC biology and clinical applications.
In 2020, it topped the list of most diagnosed cancers. Historical disparities in mortality rates are linked to variable access to diagnosis, treatment, and care. Epidemiological trends show that while high-income countries report significant mortality declines, low- and middle-income countries face stagnant or increasing rates. The World Health Organization's Global Breast Cancer Initiative (GBCI) aims to address these disparities through three strategic pillars: promoting early detection, ensuring timely diagnosis and treatment, and providing comprehensive care. The initiative's goals include diagnosing most new cases at early stages, completing diagnostics within 60 days, and ensuring comprehensive treatment for over 80% of patients. This approach not only targets health improvement but also strengthens health systems under the broader cancer control framework.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for COVID-19, has been linked to a range of neurological symptoms and complications beyond its primary respiratory effects. This review summarizes current literature on the neurotropic mechanisms and neurological manifestations of COVID-19, highlighting the need for healthcare professionals to detect and address these symptoms to mitigate long-term consequences.
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