Article
Anatomy & Morphology
Dmitry Bagrov, Grigory S. Glukhov, Andrey Moiseenko, Maria G. Karlova, Daniil S. Litvinov, Petr A. Zaitsev, Liubov Kozlovskaya, Anna A. Shishova, Anastasia A. Kovpak, Yury Y. Ivin, Anastasia N. Piniaeva, Alexey S. Oksanich, Viktor P. Volok, Dmitry Osolodkin, Aydar A. Ishmukhametov, Alexey M. Egorov, Konstantin Shaitan, Mikhail P. Kirpichnikov, Olga S. Sokolova
Summary: This study characterized the beta-propiolactone inactivated SARS-CoV-2 virions using TEM and AFM, finding that samples prepared using SEC and IEC retained more spikes on the surface and the S proteins were in the pre-fusion conformation. Analytical TEM showed that the inactivated virions retained nucleic acid, demonstrating their potential as a vaccine candidate.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Review
Cell Biology
Yesim Bilmez, Gunel Talibova, Saffet Ozturk
Summary: Histone methylation is a crucial epigenetic mechanism for regulating gene expression. KMTs and PRMTs catalyze the methylation of lysine and arginine residues, while HDMs can reversibly remove the added methyl groups.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Denis Fougerouse, David W. Saxey, William D. A. Rickard, Steven M. Reddy, Rick Verberne
Summary: Well-defined reconstruction parameters are crucial for quantifying nanoscale features in atom probe tomography datasets. Testing various approaches, it was found that constraint length measurement of evaporated material provides more consistent results than tip radius measurement. Standardized parameters can alleviate potential problems in measuring nanoscale features caused by inappropriate parameters.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Anatomy & Morphology
Gul Lalay, Sami Ullah, Iftikhar Ahmed
Summary: The study found that the application of drought-tolerant Pseudomonas sp., Staphylococcus sp., and biochar of Morus alba L. wood can significantly improve the growth and physiological characteristics of Brassica napus L. plants under drought stress conditions. This synergistic application enhanced plant antioxidant enzyme activity and increased photosynthetic pigment content, showing potential for alleviating the adverse effects of drought stress on crop yields.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Materials Science, Multidisciplinary
Matthew Olszta, Derek Hopkins, Kevin R. Fiedler, Marjolein Oostrom, Sarah Akers, Steven R. Spurgeon
Summary: This article discusses the design of a closed-loop instrument control platform guided by emerging sparse data analytics. It proposes that a centralized controller informed by machine learning can drive on-the-fly experimental decision-making, enabling practical analysis of various material features using automated microscopy.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Microscopy
Joshua L. Dickerson, Peng-Han Lu, Dilyan Hristov, Rafal E. Dunin-Borkowski, Christopher J. Russo
Summary: This study investigates the potential improvements in using electron cryomicroscopy to image thick specimens with high-resolution phase contrast imaging. The research demonstrates that chromatic aberration correction can enhance the imaging of thick biological samples. The study also suggests that with advancements in transmission electron microscope technology, it may be possible to directly locate and identify sub-100 kDa proteins in vitrified specimens taken from cells without the need for labels.
Article
Microscopy
Baokai Wang, Qiming Zhang, Xi Chen, Haitao Luan, Min Gu
Summary: Fibre-optical microendoscopy, based on fibre-optical confocal scanning microscopy, has brought innovations in imaging, diagnostics, and microsurgery. The lens system plays a significant role in resolution and miniaturization.
JOURNAL OF MICROSCOPY
(2022)
Article
Anatomy & Morphology
Rafia Attique, Muhammad Zafar, Mushtaq Ahmad, Sadia Zafar, Muhammad Asad Ghufran, Muhammad Raza Ul Mustafa, Ghulam Yaseen, Latif Ahmad, Shazia Sultana, Nabila, Aqeela Zafar, Salman Majeed
Summary: This research explores the morphology and palynological features of melliferous bee flora pollen from arid zones in District Attock, Islamabad Capital and Rawalpindi. The study demonstrates the significance of pollen morphology in correctly identifying honeybee flora species.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Review
Cell Biology
Hidenobu Miyaso, Yuki Ogawa, Masahiro Itoh
Summary: The male reproductive system includes the testes, ducts, accessory sex glands, and penis. Active research has identified various biological factors that regulate germ cell development and function, including genetics, epigenetics, and the neuroendocrine-immune system. This article reviews these factors and their impact on the microenvironment of germ cells, with a focus on the immunoendocrine environment.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Review
Microscopy
Tiara V. Hinton, Sharon Batelu, Noah Gleason, Timothy L. Stemmler
Summary: Fe-S clusters play crucial roles in biochemical pathways in living organisms, synthesizing through mitochondrial and cytosolic pathways in eukaryotes. Deficiency in Fe-S clusters can be detrimental to cell and organism viability.
Article
Anatomy & Morphology
Kainat Kanwal, Muhammad Zafar, Amir Muhammad Khan, Tariq Mahmood, Qamar Abbas, Fethi Ahmet Ozdemir, Mushtaq Ahmad, Shazia Sultana, Rozina, Anam Fatima, Aqsa Aziz, Anmol Niazi
Summary: The search for renewable and alternative energy resources is a fiercely disputed problem in the scientific world due to the increase in energy catastrophe and accompanying environmental issues. Biodiesel has proven to be the finest sustainable alternative to gasoline, with nonedible oil-bearing seeds potentially being a cost-effective and environment-friendly resource for biodiesel production. The use of scanning electron microscopy (SEM) to investigate micromorphological features of novel nonedible oil-bearing seeds of Verbenaceae for biodiesel feed stocks shows promise in aiding future research, seed identification, and authenticity.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Anatomy & Morphology
Seema Gul, Kanwar Rehan Latafat, Maliha Asma, Mushtaq Ahmad, Zeyneb Kilic, Muhammad Zafar, Yifu Ding, Aamir Malik
Summary: The study demonstrates the synthesis of zirconium-based MOFs, UiO-66 and UiO-66-SO3H, with a mean diameter of 200 nm and their intercalation into PES substrate to fabricate TFN membranes via IP method. TFN membranes show higher selectivity and permeability compared to TFC membranes, especially for heavy metals such as Cd and Hg.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Materials Science, Multidisciplinary
Berit H. Goodge, Ismail El Baggari, Seung Sae Hong, Zhe Wang, Darrell G. Schlom, Harold Y. Hwang, Lena F. Kourkoutis
Summary: This paper introduces a phase demodulation technique for studying structural order in materials, which extracts important information about local variations and heterogeneities in crystalline lattices. The technique can be used to analyze defects, dislocations, and superlattice structures in crystals.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Anatomy & Morphology
Rehman Ullah, Safia Sher, Zahir Muhammad, Saiqa Afriq Jan, Muhammad Nafees
Summary: The study aimed to modulate the physiological response of sunflowers in a stressed environment using Urea capped hydroxyapatite nanoparticles (Urea-HANPs). Results revealed that Urea-HANPs significantly improved the plant's resistivity to osmotic stress and enhanced agronomic and physiobiochemical attributes.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Anatomy & Morphology
Ali Bahadur, Shahid Iqbal, Hashem O. Alsaab, Nasser S. Awwad, Hala A. Ibrahium
Summary: The study introduced a new method of degrading PMMA materials using AlI3 NAs. The results showed that AlI3 NAs can enhance the stability of polymers and effectively retard the combustion rate of polymers.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Microscopy
Meng-jia Li, Shuo Liu, Xiang-dong Wang, Yun-jia Shi, Qing-lin Pan, Xiao-jie Zhou, Rui-feng Zhang, Nick Birbilis
Summary: The influence of Sc and Zr on sensitization and intergranular corrosion in Al-5.8Mg-0.4Mn alloy was investigated. Results show that the addition of Sc and Zr improves the strength and intergranular corrosion resistance of the alloy. The presence of Al-3(Sc1-xZrx) dispersoids alters the proportion of low angle grain boundaries and decreases the volume fraction of beta-phase in the alloy.
Review
Anatomy & Morphology
Hannah A. Bullock, Cynthia S. Goldsmith, Sara E. Miller
Summary: Identifying coronavirus particles by electron microscopy is challenging due to the need for specific knowledge of viral ultrastructure and replication, as well as familiarity with normal subcellular organelles. To counter the misidentification of subcellular structures as coronavirus particles in tissue specimens, it is important to understand the morphological features that differentiate them. Additionally, ultrastructural changes observed in tissues from SARS-CoV-2-infected individuals may indicate the widespread effects of the infection on the body, either through direct viral infection or inflammatory and immune processes.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Microscopy
Bo Ren, Rui-feng Zhao, Ai-yun Jiang, Yuan Yu
Summary: The effect of Cr content on the microstructure and oxidation behavior of high-entropy alloys was investigated. Increasing Cr content resulted in increased elemental segregation and improved oxidation resistance for phases rich in Cr.
Article
Microscopy
Yves Auad, Michael Walls, Jean-Denis Blazit, Odile Stephan, Luiz H. G. Tizei, Mathieu Kociak, Francisco De la Pena, Marcel Tence
Summary: The study developed an event-based hyperspectral EELS technique using a specific integrated circuit detector and embedded time-to-digital lines, allowing for hyperspectral image acquisition without additional costs, as well as enabling the study of chemical reactions in samples under electron beam irradiation.
Article
Microscopy
James F. Nohl, Nicholas T. H. Farr, Yige Sun, Gareth M. Hughes, Serena A. Cussen, Cornelia Rodenburg
Summary: Powder materials are widely used in various fields of materials science, and scanning electron microscopy (SEM) has been developed to characterize their morphological, microstructural, and bulk chemical properties. However, SEM currently lacks the ability to provide spatially localized chemical information on powder surfaces with resolution similar to secondary electron (SE) imaging. This review discusses the potential of using energy filtered SEM for surface chemical analysis using SE hyperspectral imaging (SEHI) and compares it with other commonly used chemical characterization methods. The applicability of existing powder preparation methods for SEM to SEHI is also reviewed. The review includes examples of SEHI characterization of carbon-fiber/polyamide composite powder and lithium nickel cobalt oxide (NMC) powder surfaces, demonstrating the ability of SEHI to differentiate bonding and extract information about surface work function.
