Article
Biochemistry & Molecular Biology
Priyanga Kumar, Noel Nesakumar, Jayashree Gopal, Sakthivel Sivasubramanian, Srinivasan Vedantham, John Bosco Balaguru Rayappan
Summary: This study developed an electrochemical biosensor for measuring MG levels in the plasma of type-2 diabetes mellitus patients. The biosensor showed a wide linear concentration range, high sensitivity, and a 90% correlation with ELISA data. It also exhibited a significant correlation with HbA1c and fasting plasma glucose levels.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yu Zhao, Jian Zhang, Nan Ma, Jinming Kong, Xueji Zhang
Summary: In this study, a vitamin B12-based electrochemical biosensor was developed for early screening of lung cancer. The method achieved high sensitivity detection of the lung cancer biomarker miRNA-21 at the femtomolar level using atom transfer radical polymerization.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Milutin Smiljanic, Pierre Bleteau, Alexia Papageorgiou, Nathan Goffart, Catherine Adam, Thomas Doneux
Summary: The electrochemical properties of three oxazine fluorophores, ATTO655, ATTO680 and ATTO700, were investigated on gold electrodes. These fluorophores exhibit reversible or quasi-reversible voltammetric behavior depending on the pH. ATTO655, in particular, showed promising performance as a redox label for electrochemical nucleic acid sensing without affecting DNA folding. Hybridization experiments showed significant signal variation between single-stranded DNA and double-stranded DNA.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Lan Wang, Hongmei Yuan, Wenwen Li, Peishuo Yan, Mengxia Zhao, Zhongzheng Li, Huabin Zhao, Shenghui Wang, Ruyan Wan, Yajun Li, Juntang Yang, Xin Pan, Ivan Rosas, Guoying Yu
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal lung disease of unknown cause. Decreased expression of ACSS3 in IPF patients and mice with fibrosis was found, leading to the accumulation of lipid droplets and increased glycolysis. ACSS3 regulates the activity of alveolar epithelial cells through metabolic reprogramming, potentially serving as a therapeutic target for pulmonary fibrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Yanling Zhang, Jie Wang, Min Liu, Yun Ni, Yao Yue, Dawei He, Ruijiang Liu
Summary: An innovative electrochemical biosensor has been developed for sensitive detection of HER2 protein, an important tumor marker for breast cancer diagnosis and treatment evaluation. The biosensor utilizes specific recognition probes and nanomaterials, along with a unique sensing strategy, to achieve ultra-low detection limit and demonstrate excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Chhanda Bose, Sudhir Kshirsagar, Murali Vijayan, Subodh Kumar, Sharda P. Singh, Ashly Hindle, P. Hemachandra Reddy
Summary: This study investigates the role of RLIP76 in Alzheimer's Disease (AD) by analyzing brain tissues from AD patients and control subjects. The findings show a gradual decrease in the expression of RLIP76 protein with AD progression, and its co-localization with Amyloid beta and phosphorylated tau. AD patients exhibited higher levels of lipid peroxidation, H2O2 production, and oxidative DNA damage, as well as lower ATP production compared to controls. COMET assay revealed numerous DNA strand breaks in AD patients. These results indicate that RLIP76 plays an important role in maintaining neuronal health and homeostasis, and could be a promising therapeutic target for Alzheimer's disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Yun Zhang, Shiqiang Li, Chenxu Peng, Zhe Shi, Xiangmin Miao
Summary: In this study, a method based on Au@luminol nanoparticles was established for the sensitive detection of miRNA-21 in cancer cell-derived exosomes. The method showed enhanced electrochemiluminescence and achieved sensitive detection with a detection limit of 0.43 fM. It demonstrated potential application value in early clinical diagnosis.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Valentin Larzilliere, Florence de Fouchecour, Chrystelle Bureau, Theodore Bouchez, Roman Moscoviz
Summary: This study aimed to evaluate the long-term influence of inoculation strategies on bioelectrochemical systems for wastewater treatment and found that different strategies did not have a significant long-term impact on system performance and microbial compositions after three batches.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Kamal Asadipour, Maisoun Bani Hani, Lucas Potter, Brittney L. Ruedlinger, Nicola Lai, Stephen J. Beebe
Summary: This study demonstrates that nanosecond pulsed electric fields (nsPEFs) can modulate cellular redox functions and metabolism by regulating trans-plasma membrane electron transport and mitochondrial electron transport system. The effects of nsPEFs on cells are dose-dependent, with low levels being beneficial and high levels causing damage.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
M. Seco-Cervera, D. Ortiz-Masia, D. C. Macias-Ceja, S. Coll, L. Gisbert-Ferrandiz, J. Cosin-Roger, C. Bauset, M. Ortega, B. Heras-Moran, F. Navarro-Vicente, M. Millan, J. V. Esplugues, S. Calatayud, M. D. Barrachina
Summary: The study revealed the presence of resistance to apoptosis in complicated ileal Crohn's disease, with PDGFB inducing an ETS1-mediated resistance to apoptosis associated with an inflammatory and fibrogenic pattern of expression in intestinal fibroblasts. Potential targets against ileal fibrosis include PDGFRB, IL1R1, or MCL1.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Paula Sanchon-Sanchez, Elisa Herraez, Rocio I. R. Macias, Maria C. Estiu, Puri Fortes, Maria J. Monte, Jose J. G. Marin, Marta R. Romero
Summary: This study investigated the relationship between cholestasis and the expression of crucial enzymes involved in progesterone metabolism in the liver and placenta. The results showed that cholestasis markedly altered the expression of these enzymes, with a more significant effect in the placenta than in the liver. These findings suggest that the excess of progesterone metabolites in the serum of ICP patients can involve both enhanced placental production and decreased hepatic clearance.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Biochemistry & Molecular Biology
Yunmeng Wang, Ping Cheng
Summary: Oncolytic viruses (OVs) are emerging as therapeutically relevant anticancer agents, especially when combined with genetically modified bispecific T cell engagers (BiTEs). This combination strategy can overcome the limitations of BiTEs alone and provide targeted cytotoxicity to solid tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Mengxin Li, Xuanzhong Wang, Xuyang Chen, Jinghui Hong, Ye Du, Dong Song
Summary: Pancreatic adenocarcinoma (PAAD) is a common digestive malignant tumor with limited treatment options. This study demonstrates that TGM2 may serve as a marker for treatment and prognosis in pancreatic cancer patients. Co-treatment of low dose cisplatin (DDP) and the TGM2 inhibitor GK921 effectively inhibits PAAD cell viability and proliferation in vitro and in vivo, by inhibiting epithelial-to-mesenchymal transition (EMT) induced by TGM2 and enhancing cell cycle arrest and apoptosis caused by DDP. These findings suggest that the combination of GK921 and DDP holds promise as a treatment for PAAD patients.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Zi-Ran Kang, Shanshan Jiang, Ji-Xuan Han, Yaqi Gao, Yile Xie, Jinxian Chen, Qiang Liu, Jun Yu, Xin Zhao, Jie Hong, Haoyan Chen, Ying-Xuan Chen, Huimin Chen, Jing-Yuan Fang
Summary: The study demonstrates that BCAA metabolism is involved in the development of colorectal cancer (CRC). BCAT2 deficiency promotes CRC progression by inhibiting BCAA metabolism and chronically activating the mTORC1 pathway.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Sijie Yin, Zhiqiang Yu, Ningning Song, Zhanjun Guo, Wei Li, Jinhang Ma, Xiaoyan Wang, Junyao Liu, Minmin Liang
Summary: Researchers have developed a microneedle-based glucose monitoring skin patch that can continuously and accurately monitor glucose levels in real time, offering high sensitivity and a long lifespan. The results can be displayed on a smartphone.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minsu Jang, Jonghoon Shin, You Hwan Kim, Tae-Young Jeong, Soojin Jo, Sung-Jo Kim, Vasanthan Devaraj, Joonhee Kang, Eun-Jung Choi, Ji Eun Lee, Jin-Woo Oh
Summary: Metabolome analysis using 3D superstructures has been applied for glaucoma diagnosis, providing comprehensive information and accurate classification of patients. The advantages of 3D superstructures, including high hotspot density, excellent signal repeatability, and thermal stability, enable versatile metabolic analysis and disease diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lanlan Xu, Mo Ma, Jingkang Li, Dianfeng Dai, Dejiang Gao, Pinyi Ma, Qiong Wu, Daqian Song
Summary: This study developed an ultra-sensitive probe, LAN-apn, to investigate the expression level of APN in thyroid cancer and evaluate its potential as a biomarker. The results suggest that APN may become a new biomarker for thyroid cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
