Journal
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
Volume 94, Issue 11, Pages 1603-1607Publisher
FERRATA STORTI FOUNDATION
DOI: 10.3324/haematol.2009.007708
Keywords
interleukin-18; interleukin-18 binding protein; high-dose dexamethasone; idiopathic thrombocytopenic purpura
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Funding
- National Natural Science Foundation of China [30600259, 30600680, 30770922, 30800491, 30801258]
- 973 Program [2006 CB 503803]
- National Excellent Doctoral Dissertation of PR China [200561]
- New Century Excellent Talents in University [NCET-07-0514]
- Key Project of Chinese Ministry of Education [109097]
- Key Clinical Research Project of Public Health Ministry of China
- Commonweal Trade for Scientific Research [200802031]
- Taishan
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To evaluate the effects of high-dose dexamethasone (HD-DXM) on the balance of interleukin-18 (IL-18) and its endogenous antagonist IL-18 binding protein (IL-18BP) in ITP patients, IL-18, IL-18BP as well as IFN-gamma, IL-4 plasma levels and platelet counts were determined in 17 ITP patients receiving DXM 40 mg/day for four consecutive days and in 24 healthy subjects. Using RT-PCR, the mRNA expression of IL-18, IL-18BP, IFN-gamma, IL-4, T-box (T-bet) and GATA-binding protein 3(GATA-3) were studied in all subjects. The in vitro effects of DXM on IL-18BP and IL-18 of peripheral blood mononuclear cells (PBMCs) were studied by ELISA. HD-DXM administration increased IL-18BP and reduced IL-18 expression significantly (p<0.05), which resulted in a downregulation of IL-18/IL-18BP ratio p<0.05). In vitro, DXM had a significant effect on secretion of IL-18BP while diminishing IL-18 release from cultures of PBMCs. These results suggest that downregulation of IL-18/IL-18BP might account for its clinical efficacy of HDDXM in active ITP.
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