Journal
GUT
Volume 59, Issue 5, Pages 612-621Publisher
BMJ PUBLISHING GROUP
DOI: 10.1136/gut.2009.190439
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Funding
- Crohn's and Colitis Foundation of Canada (CCFC)
- GIDRU CIHR
- Canadian Institutes of Health Research
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Objectives Abdominal pain is a serious cause of morbidity in patients with inflammatory bowel disease. To better understand the mechanisms and potentially identify new targets for treatment, the effects of inflammatory supernatant from colonic biopsies of patients with active ulcerative colitis (UC) on mouse colonic nociceptive dorsal root ganglia neurons were examined. Methods Acutely dissociated dorsal root ganglia neurons innervating the mouse colon were incubated in supernatants obtained from colonic biopsies from patients with UC. Whole-cell patch clamp recordings were obtained to examine the effects on neuronal excitability. The role of tumour necrosis factor alpha (TNF alpha) was studied using TNF alpha receptor (TNFR) knockout mice and comparing supernatant and TNF alpha actions. Results UC supernatants significantly decreased the rheobase and increased action potential discharge, indicating increased neuronal excitability. Human biopsies exhibited high levels of TNF alpha, and mouse colonic neurons only exhibited TNFR1 mRNA. Incubation with TNF alpha recapitulated the supernatant effects on neuronal excitability, and supernatant and TNF alpha actions were almost completely blocked in TNFR knockout mice. In voltage clamp studies, transient I-A and I-K currents were suppressed and Na-v 1.8 currents were enhanced by TNF alpha and UC supernatant, suggesting that multiple underlying mechanisms contributed to the enhanced excitability. Conclusions UC supernatants enhance neuronal excitability of sensory dorsal root ganglia neurons innervating the colon. TNF alpha is a key mediator which acts at neuronal TNFR1 to modulate K-v and Na-v currents. Together these data provide a number of potential new targets for pain management in UC.
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