Journal
GUT
Volume 59, Issue 3, Pages 311-319Publisher
BMJ PUBLISHING GROUP
DOI: 10.1136/gut.2009.183608
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Funding
- Coeliac UK
- Rothschild Trust Corporation and Associazione Italiana Celiachia Regione Puglia [1400/07]
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Background An unresolved question in coeliac disease is to understand how some toxic gliadin peptides, in particular p31-43, can initiate an innate response and lead to tissue transglutaminase (TG2) upregulation in coeliac intestine and gliadin sensitive epithelial cell lines. Aim We addressed whether the epithelial uptake of p31-43 induces an intracellular pro-oxidative envoronment favouring TG2 activation and leading to the innate immune response. Methods The time course of intracellular delivery to lysosomes of p31-43, p alpha-2 or p alpha-9 gliadin peptides was analysed in T84 and Caco-2 epithelial cells. The effects of peptide challenge on oxidative stress, TG2 and peroxisome proliferator-activated receptor ( PPAR)gamma ubiquitination and p42/44-mitogen activated protein ( MAP) kinase or tyrosine phosphorylation were investigated in cell lines and cultured coeliac disease biopsies with/without anti-oxidant treatment or TG2 gene silencing by immunoprecipitation, western blot, confocal microscopy and Fluorenscence Transfer Resonance Energy ( FRET) analysis. Results After 24 h of challenge p31-43, but not p alpha-2 or p alpha-9, is still retained within LAMP1-positive perinuclear vesicles and leads to increased levels of reactive oxygen species (ROS) that inhibit TG2 ubiquitination and lead to increases of TG2 protein levels and activation. TG2 induces cross-linking, ubiquitination and proteasome degradation of PPAR gamma. Treatment with the antioxidant EUK-134 as well as TG2 gene silencing restored PPAR gamma levels and reversed all monitored signs of innate activation, as indicated by the dramatic reduction of tyrosine and p42/p44 phosphorylation. Conclusion p31-43 accumulation in lysosomes leads to epithelial activation via the ROS-TG2 axis. TG2 works as a rheostat of ubiquitination and proteasome degradation and drives inflammation via PPAR gamma downregulation.
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