Journal
KIDNEY INTERNATIONAL
Volume 87, Issue 5, Pages 975-983Publisher
ELSEVIER SCIENCE INC
DOI: 10.1038/ki.2014.394
Keywords
beta-catenin signaling; CAKUT; gestational hypoxia; kidney development; nephrogenesis; nephron number
Categories
Funding
- National Health and Medical Research Council of Australia [APP1009338]
- [APP631361]
- [APP1042093]
- [APP1042002]
Ask authors/readers for more resources
Gestational stressors, including glucocorticoids and protein restriction, can affect kidney development and hence final nephron number. Since hypoxia is a common insult during pregnancy, we studied the influence of oxygen tension on kidney development in models designed to represent a pathological hypoxic insult. In vivo mouse models of moderate, transient, midgestational (12% O-2, 48 h, 12.5 dpc) or severe, acute, early-gestational (5.5-7.5% O-2, 8 h, 9.5-10.5 dpc) hypoxia were developed. The embryo itself is known to mature under hypoxic conditions with embryonic tissue levels of oxygen estimated to be 5%-8% (physiological hypoxia) when the mother is exposed to ambient normoxia. Both in vivo models generated phenotypes seen in patients with congenital anomalies of the kidney and urinary tract (CAKUT). Severe, acute, early hypoxia resulted in duplex kidney, while moderate, transient, midgestational hypoxia permanently reduced ureteric branching and nephron formation. Both models displayed hypoxia-induced reductions in beta-catenin signaling within the ureteric tree and suppression of the downstream target gene, Ccnd1. Thus, we show a link between gestational hypoxia and CAKUT, the phenotype of which varies with timing, duration, and severity of the hypoxic insult.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available