Journal
GROWTH HORMONE & IGF RESEARCH
Volume 22, Issue 2, Pages 53-58Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.ghir.2011.12.005
Keywords
Growth hormone; Doping; Test; Insulin-like growth factor-I (IGF-I); Amino-terminal pro-peptide of type III collagen (P-III-NP)
Categories
Funding
- World Anti-Doping Agency (WADA)
- UK Anti-Doping
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Background: The GH-2000 project developed a method for detecting GH misuse based on the measurement of insulin-like growth factor-I (IGF-I) and the amino-terminal pro-peptide of type III collagen (P-III-NP). The objective of this study was to develop decision limits for the GH-2000 score to detect GH misuse in elite athletes using two currently available commercial assays for each analyte. Study design: Subjects: 404 male (mean age 23.9 yrs, range 12-37 yrs) and 94 female elite athletes (mean age 24.5 yrs, range 18-34 yrs) participated. Blood samples were collected according to World Anti-Doping Agency (WADA) guidelines at various sporting events including 238 samples collected as part of the UK Anti-Doping Testing Programme. Laboratory analysis: IGF-I was measured by Siemens Immulite IGF-I assay and Immunotech A15729 IGF-I IRMA. P-III-NP was measured by RIA-gnost P-III-P and the UniQ (TM) PIIINP RIA. Statistical analysis: The GH-2000 score decision limits were developed through the analysis of the elite athlete samples. Results: For males and females separately, the distributions of GH-2000 scores were consistent with Normal distributions. Using a specificity of 99.99% new decision limits were determined which included an allowance for uncertainty associated with calculations based on a finite sample size. One outlier was identified with results incompatible with normal physiology and tested positive with the current isoform GH test. Conclusions: We have developed decision limits using currently available commercial assays to measure IGF-I and P-III-NP in elite athletes. This should allow the introduction of a test for CH misuse based on the measurement of these GH sensitive biomarkers. (C) 2012 Elsevier Ltd. All rights reserved.
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