Prodomains regulate the synthesis, extracellular localisation and activity of TGF-β superfamily ligands

All transforming growth factor-beta (TGF-beta) ligands are synthesised as precursor molecules consisting of a signal peptide, an N-terminal prodomain and a C-terminal mature domain. During synthesis, prodomains interact non-covalently with mature domains, maintaining the molecules in a conformation competent for dimerisation. Dimeric precursors are cleaved by proprotein convertases, and TGF-beta ligands are secreted from the cell non-covalently associated with their prodomains. Extracellularly, prodomains localise TGF-beta ligands within the vicinity of their target cells via interactions with extracellular matrix proteins, including fibrillin and perlecan. For some family members (TGF-beta 1, TGF-beta 2, TGF-beta 3, myostatin, GDF-11 and BMP-10), prodomains bind with high enough affinity to suppress biological activity. The subsequent mechanism of activation of these latent TGF-beta ligands varies according to cell type and context, but all activating mechanisms directly target prodomains. Thus, prodomains control many aspects of TGF-beta superfamily biology, and alterations in prodomain function are often associated with disease.