Journal
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
Volume 249, Issue 4, Pages 529-536Publisher
SPRINGER
DOI: 10.1007/s00417-010-1573-9
Keywords
Apoptosis; Advanced glycation end products; Diabetic keratopathy
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Funding
- Korea Institute of Oriental Medicine (KIOM) [L08010, K09030]
- Ministry of Education, Science & Technology (MoST), Republic of Korea [L08010] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Council of Science & Technology (NST), Republic of Korea [K09030] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Background The purpose of the experiment reported here was to assess the involvement of advanced glycation end products (AGEs), oxidative stress, and nuclear factor kappa-B (NF-kappa B) activation in the development of diabetic keratopathy. Methods Diabetes was induced by intraperitoneal streptozotocin injection in male Sprague-Dawley rats. The thickness of the cornea was measured. Apoptosis was detected by TUNEL assay and western blot for caspase-3. The expression of AGEs and 8-hydroxydeoxyguanosine (8-OHdG) were studied by immunohistochemistry in corneal tissues of normoglycaemic and diabetic rats. NF-kappa B activation was evaluated by electrophoretic mobility shift assay and southwestern histochemistry. Results Corneal edema was observed in diabetic rats. The thickness of cornea was higher in diabetic than in control rats. AGEs were accumulated in corneal tissues. 8-OHdG and NF-kappa B were identified in corneal epithelium, stroma and endothelium, and its expressions were greater in diabetic than in those of control rats. Diabetes induces significant alterations in rat corneal tissue structure. Conclusions The higher expression of AGE, 8-OHdG and NF-kappa B in corneal tissues of diabetic rats suggests that these factors are involved in apoptosis and in subsequent corneal alterations related to diabetic keratopathy.
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