Journal
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
Volume 246, Issue 8, Pages 1117-1122Publisher
SPRINGER
DOI: 10.1007/s00417-008-0845-0
Keywords
Cre recombinase; Rosa26 Cre reporter; anterior eye segment; Cre-loxP system; transgenic mice
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Funding
- Grants-in-Aid for Scientific Research [19679008] Funding Source: KAKEN
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Purpose To map neural crest cell fate during eye development. Methods Neural crest cells were tracked in developing mouse eyes using a transgene expressing Cre recombinase controlled by the Protein 0 promoter and a Rosa26 Cre-responsive reporter gene that produced beta-galactosidase after Cre-mediated recombination. Results beta-galactosidase-positive cells were detected in the periocular segment on embryonic day (E) 9.5. Several neural crest cell-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were strongly stained on E13.5-E18.5. The staining decreased in the corneal stroma after birth, but persisted in the presumptive iridocorneal angle. Conclusions Protein 0-Cre transgenic mice offer a conditional knock-out strategy to investigate anterior eye segment differentiation.
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