Journal
GLYCOCONJUGATE JOURNAL
Volume 30, Issue 5, Pages 537-547Publisher
SPRINGER
DOI: 10.1007/s10719-012-9453-7
Keywords
Alpha1-antitrypsin; N-glycan; In vivo half-life; Sialic acid; Antennary structure; Chinese hamster ovary (CHO) cells
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Funding
- Rural Development Administration [SSAC-PJ008001]
- Korea Research Council of Fundamental Science and Technology (KRCF)
- Korea Small and Medium Business Administration [SA113037]
- Korea Evaluation Institute of Industrial Technology (KEIT) [SA113037] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Human alpha-1-antitrypsin (alpha 1AT) is a glycoprotein with protease inhibitor activity protecting tissues from degradation. Patients with inherited alpha 1AT deficiency are treated with native alpha 1AT (nAT) purified from human plasma. In the present study, recombinant alpha 1AT (rAT) was produced in Chinese hamster ovary (CHO) cells and their glycosylation patterns, inhibitory activity and in vivo half-life were compared with those of nAT. A peptide mapping analysis employing a deglycosylation reaction confirmed full occupancy of all three glycosylation sites and the equivalency of rAT and nAT in terms of the protein level. N-glycan profiles revealed that rAT contained 10 glycan structures ranging from bi-antennary to tetra-antennary complex-type glycans while nAT displayed six peaks comprising majorly bi-antennary glycans and a small portion of tri-antennary glycans. In addition, most of the rAT glycans were shown to have only core alpha(1 - 6)-fucose without terminal fucosylation, whereas only minor portions of the nAT glycans contained core or Lewis X-type fucose. As expected, all sialylated glycans of rAT were found to have alpha(2 - 3)-linked sialic acids, which was in sharp contrast to those of nAT, which had mostly alpha(2 - 6)-linked sialic acids. However, the degree of sialylation of rAT was comparable to that of nAT, which was also supported by an isoelectric focusing gel analysis. Despite the differences in the glycosylation patterns, both alpha 1ATs showed nearly equivalent inhibitory activity in enzyme assays and serum half-lives in a pharmacokinetic experiment. These results suggest that rAT produced in CHO cells would be a good alternative to nAT derived from human plasma.
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