Journal
GLYCOCONJUGATE JOURNAL
Volume 26, Issue 5, Pages 597-607Publisher
SPRINGER
DOI: 10.1007/s10719-008-9201-1
Keywords
Plant; N-glycosylation; Glucosidase II; Glucose-trimming
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Funding
- Centre National de la Recherche Scientifique (CNRS)
- French Ministere de la Recherche to VG and by an Australian Research Council
- MIIAT-BP
- CNRS [VG191063200]
- NIH (USA) [GM044500]
- Howard Hughes Medical Institute
- National Agency for the Promotion of Science and Technology
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Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of alpha and beta subunits. Here we present the first characterization of a plant glucosidase II alpha subunit at the molecular level. Expression of the Arabidopsis alpha subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe alpha- or alpha beta-deficient mutants, but with a lower efficiency in the last case. Inactivation of the alpha subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
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