Journal
GLYCOCONJUGATE JOURNAL
Volume 26, Issue 8, Pages 935-944Publisher
SPRINGER
DOI: 10.1007/s10719-008-9131-y
Keywords
Sialic acids; O-acetylated; O-Acetyltransferases; O-Acetylesterases; Thin-layer chromatography; High-performance liquid chromatography; Radiometry; Colorimetry
Categories
Funding
- Deutsche Forschungsgemeinschaft [Scha 202/33-1]
- Sialic Acids Society, Kiel
Ask authors/readers for more resources
The O-acetylation of sialic acids is one of the most frequent modifications of these monosaccharides and modulates many cell biological and pathological events. Sialic acid-specific O-acetyltransferases and O-acetylesterases are responsible for the metabolism of esterified sialic acids. Assays were developed for the analysis of the activities and specificities of these enzymes. The methods had to be varied in dependence on the substrate assayed, the kind of biological source, and the state of enzyme purity. With the new techniques the primary site of O-acetyl incorporation at C-7, catalyzed by the animal sialate-O-acetyltransferases studied, was ascertained. Correspondingly, this enzyme, for example from bovine submandibular gland, can be denominated as AcCoA:sialate-7-O-acetyltransferase (EC 2.3.1.45). Methods for assaying the activity of esterases de-O-acetylating sialic acids and their metabolic cooperation with the O-acetyltransferases are presented.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available