Journal
GLIA
Volume 61, Issue 7, Pages 1146-1154Publisher
WILEY
DOI: 10.1002/glia.22505
Keywords
RG-like cell; AMPA receptor; GABAA receptor; dentate gyrus; adult neurogenesis
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Funding
- German Research Foundation [SFB/TR3, SPP1172]
- European Community (NeuroGlia, ESF EuroEPINOMICS) [FP7-202167]
- Jason Brice Foundation [JBF-20101AS]
- Medical Research Council [G0801418B, G0300356, G0801418] Funding Source: researchfish
- MRC [G0801418, G0300356] Funding Source: UKRI
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Glial fibrillary acidic protein (GFAP)-positive astrocytes with radial processes [radial glia (RG)-like cells] in the postnatal dentate gyrus share many of the characteristics of embryonic radial glia and appear to act as precursor cells for adult dentate neurogenesis, a process important for pattern separation and hippocampus-dependent learning. Although much work has delineated the mechanisms underlying activity-neurogenesis coupling via gamma-amino butyric acid (GABA)ergic neurotransmission on GFAP-negative transient-amplifying cells and neuroblasts, little is known regarding the effects of neurotransmitters on RG-like cells. Conflicting evidence exists for both GABA and glutamate receptors on these cells. Here, using GFAP reporter mice, we show that the somatic membrane of RG-like cells carries GABAA receptors and glutamate transporters but not ionotropic glutamate receptors, whereas 2-amino-3-(hydroxyl-5-methylisoxazole-4-yl) propionic acid (AMPA) and GABAA receptors are expressed on the processes of these cells. Almost all RG-like cells expressed the GluA2 subunit, which restricts the Ca2+ permeability of AMPA receptors. The glial GABAA receptors mainly comprised 2/4, 1, and 1/3. The selective presence of AMPA receptors on the radial processes may be important for sensing and responding to local activity-driven glutamate release and supports the concept that RG-like astrocytes are composed of functional and structural domains.
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