Journal
GLIA
Volume 58, Issue 2, Pages 218-230Publisher
WILEY
DOI: 10.1002/glia.20918
Keywords
neurogenesis; astrocyte cultures; RT-PCR; subventricular zone; heterogeneity
Categories
Funding
- OLFACTOSENSE
- OLFACTOSENSE, Consortium (CAM) [P-SEM-0255-2006]
- Castilla-La Mancha Health Research Foundation [FISCAM PI2007-66]
Ask authors/readers for more resources
The rostral migratory stream (RMS) is a well defined migratory pathway for precursors of olfactory bulb (OB) interneurons Throughout the RMS an intense astroglial matrix surrounds the migratory cells. However, it is not clear to what extent the astroglial matrix participates in migration. Here, we have analyzed the migratory behavior of neuroblasts cultured on monolayers of astrocytes isolated from areas that are pet-missive (RMS and OB) and nonpermissive (cortex and adjacent cortical areas) to migration. Our results demonstrate robust neuroblast migration when RMS-explants are cultured on OB or RMS-astroclytes, in contrast to their behavior on astroglia derived from nonpermissive areas. These differences, mediated by astrocyte-derived nonsoluble factors, are related to the overexpression of extracellular matrix and cell adhesion molecules, as revealed by real-time qRT-PCR. Our results show that astroglia heterogeneity could play a significant role in migration within the RMS and in cell detachment in the OB. (C) 2009 Wiley-Liss. Inc
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available